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ABflo® 488 Rabbit anti-Human CD166/ALCAM mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | activated leukocyte cell adhesion molecule |
| Gene Symbol: | ALCAM |
| Synonyms: | MEMD; CD166 |
| Gene ID: | 214 |
| UniProt ID: | Q13740 |
| Clone ID: | 0L3I2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-526 of human CD166/ALCAM(NP_001618.2) |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ALCAM Antibody |
Background
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Images
 | Flow cytometry:1×10^6 K-562 cells (negative control,Left) and SH-SY5Y cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human CD166/ALCAM mAb(A22633,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 SH-SY5Y cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD166/ALCAM mAb(A22633,5 μl/Test,right). |
 | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD166/ALCAM mAb(A22633,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD166/ALCAM mAb(A22633,5 μl/Test,right). |
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