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ABflo® 488 Rabbit anti-Mouse CD34 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD34 antigen |
| Gene Symbol: | Cd34 |
| Synonyms: | N/A |
| Gene ID: | 12490 |
| UniProt ID: | Q64314 |
| Clone ID: | 8J4D7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-287 of mouse CD34(NP_598415.1) |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd34 Antibody |
Background
Enables sulfate binding activity. Acts upstream of or within leukocyte migration. Located in cytoplasm; external side of plasma membrane; and extracellular region. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; genitourinary system; and skin. Orthologous to human CD34 (CD34 molecule).
Images
 | Confocal imaging of NIH/3T3 cells using ABflo® 488 Rabbit anti-Mouse CD34 mAb (A23107, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry:1×10^6 Neuro-2a cells (negative control,Left) and NIH/3T3 cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD34 mAb(A23107,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry:1×10^6 NIH/3T3 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD34 mAb(A23107,5 μl/Test,right). |
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