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ABflo® 488 Rabbit anti-Mouse CD68 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | CD68 antigen |
| Gene Symbol: | Cd68 |
| Synonyms: | Lamp4; gp110; Scard1 |
| Gene ID: | 12514 |
| UniProt ID: | P31996 |
| Clone ID: | 3O4C1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-291 of mouse CD68 (NP_001277987.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd68 antibody |
Background
Involved in several processes, including cellular response to lipopolysaccharide; cellular response to oxidised low-density lipoprotein particle stimulus; and negative regulation of dendritic cell antigen processing and presentation. Acts upstream of or within aging and cellular response to organic substance. Located in lysosome and plasma membrane. Is expressed in several structures, including central nervous system; embryo mesenchyme; heart blood vessel; liver; and spleen. Orthologous to human CD68 (CD68 molecule).
Images
 | Confocal imaging of RAW 264.7 cells using ABflo® 488 Rabbit anti-Mouse CD68 mAb (A23738, dilution 1:25). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: 1×10^6 NIH/3T3 cells(negative control,left) and Raw264.7 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD68 mAb(A23738,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Raw264.7 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Mouse CD68 mAb(A23738,5 μl/Test,right). |
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