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ABflo® 594 Rabbit anti-Human Cytokeratin 19 (KRT19) mAb (100 T)
| Reactivity: | Human |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 594. Ex:588nm. Em:604nm. |
| Gene Name: | keratin 19 |
| Gene Symbol: | KRT19 |
| Synonyms: | K19; CK19; K1CS |
| Gene ID: | 3880 |
| UniProt ID: | P08727 |
| Clone ID: | 7B1O2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 301-400 of human Cytokeratin 19 (NP_002267.2). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT19 Antibody |
Background
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
Images
 | Flow cytometry: 1×10^6 Jurkat cells (negative control,left) and MCF7 cells (right) were intracellularly-stained with ABflo® 594 Rabbit anti-Human Cytokeratin 19 (KRT19) mAb(A24259,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 MCF7 were intracellularly-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Human Cytokeratin 19 (KRT19) mAb(A24259,5 μl/Test,right). |
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