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ABflo® 647 Rabbit anti-Mouse CD138/Syndecan-1 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | syndecan 1 |
| Gene Symbol: | Sdc1 |
| Synonyms: | Sstn; Synd; CD138; Synd1; syn-1 |
| Gene ID: | 20969 |
| UniProt ID: | P18828 |
| Clone ID: | 9K8Y10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-255 of mouse CD138/Syndecan-1 (NP_035649.1). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Sdc1 antibody |
Background
Predicted to enable identical protein binding activity and protein C-terminus binding activity. Involved in myoblast development. Acts upstream of or within canonical Wnt signaling pathway. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; egg cylinder; sensory organ; skin; and urinary system. Orthologous to human SDC1 (syndecan 1).
Images
 | Confocal imaging of paraffin-embedded Mouse liver tissue using ABflo® 647 Rabbit anti-Mouse CD138/Syndecan-1 mAb (A24154, dilution 1:200). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
 | Flow cytometry: 1×10^6 CHO (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse CD138/Syndecan-1 mAb (A24154,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 CHO cells (negative control,left) and CHO (Transfection,right) cells were surface-stained with ABflo® 647 Rabbit anti-Mouse CD138/Syndecan-1 mAb (A24154,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
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