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ABflo® 647 Rabbit anti-Mouse CD22 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | CD22 antigen |
| Gene Symbol: | Cd22 |
| Synonyms: | Lyb8; Lyb-8; A530093D23 |
| Gene ID: | 12483 |
| UniProt ID: | P35329 |
| Clone ID: | 10S4R1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22 (NP_033975.3). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.04 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Cd22 Antibody |
Background
Enables CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in several processes, including negative regulation of calcium-mediated signaling; negative regulation of immunoglobulin production; and regulation of B cell proliferation. Located in external side of plasma membrane and neuronal cell body membrane. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; liver and biliary system; and reproductive system. Orthologous to human CD22 (CD22 molecule).
Images
 | Confocal imaging of paraffin-embedded Mouse spleen tissue using ABflo® 647 Rabbit anti-Mouse CD22 mAb (A24628, dilution 1:100) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
 | Flow cytometry: 1×10^6 NIH/3T3 cells (negative control,left) and C57BL/6 splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD22 mAb (A24628,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 C57BL/6 splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse CD22 mAb (A24628,5 μl/Test,right). |
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