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ABflo® 647 Rabbit anti-Mouse CD45 mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | protein tyrosine phosphatase receptor type C |
| Gene Symbol: | Ptprc |
| Synonyms: | loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4 |
| Gene ID: | 19264 |
| UniProt ID: | P06800 |
| Clone ID: | 4V8E9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-566 of mouse CD45 (NP_001104786.2). |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Ptprc antibody |
Background
Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including lymphocyte differentiation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including 3rd branchial arch; alimentary system; cardiovascular system; hemolymphoid system; and placenta. Used to study systemic lupus erythematosus. Human ortholog(s) of this gene implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C).
Images
 | Confocal imaging of RAW 264.7 cells using ABflo® 647 Rabbit anti-Mouse CD45 mAb (A23708, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry:1×10^6 C2C12 cells (negative control,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD45 mAb (A23708,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry:1×10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse CD45 mAb (A23708,5 μl/Test,right). |
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