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ABflo® 647 Rabbit anti-Mouse IgE mAb (100 T)
| Reactivity: | Mouse |
| Applications: | FC (intra) |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Symbol: | immunoglobulin epsilon heavy chain constant |
| Synonyms: | IGHE,Ig epsilon chain C region |
| UniProt ID: | P06336 |
| Clone ID: | 2N5A5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 83-421 of mouse IgE(P06336). |
| Dilution: | FC (intra),5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual- |
Background
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens 1, 2. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen
Images
 | Flow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse IgE mAb(A24275,5 μl/Test,right). |
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