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ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb (100 T)
| Reactivity: | Mouse |
| Applications: | IF/ICC, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
| Gene Name: | lysosomal-associated membrane protein 1 |
| Gene Symbol: | Lamp1 |
| Synonyms: | P2B; Perk; LGP-A; CD107a; Lamp-1; LGP-120 |
| Gene ID: | 16783 |
| UniProt ID: | P11438 |
| Clone ID: | 8S3S1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 208-363 of mouse LAMP1/CD107a（NP_034814.2）. |
| Dilution: | IF/ICC,1:50 - 1:200|FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.01 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-Lamp1 antibody |
Background
Enables protein domain specific binding activity. Involved in protein stabilization. Located in several cellular components, including cytoplasmic vesicle; sarcolemma; and vacuole. Is expressed in several structures, including 1-cell stage embryo; central nervous system; craniocervical region bone; extraembryonic component; and gut. Orthologous to human LAMP1 (lysosomal associated membrane protein 1).
Images
 | Confocal imaging of C2C12 cells using ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb (A24363, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells using ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb (A24363, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of RAW 264.7 cells using ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb (A24363, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry:1×10^6 Raw264.7 cells were surface-stained with ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb(A24363,5 μl/Test,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line). Non-fluorescently stained Raw264.7 cells were used as blank control (red line). |
 | Flow cytometry:1×10^6 Raw264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or ABflo® 647 Rabbit anti-Mouse LAMP1/CD107a mAb(A24363,5 μl/Test,right). |
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