PerCP/Cyanine5.5 Rabbit anti-Mouse CD127/IL-7Rα mAb (100 T)

PerCP/Cyanine5.5 Rabbit anti-Mouse CD127/IL-7Rα mAb (100 T)

Brand: Abclonal
Cat. #: A27439
Availability: In Stock
$88.00
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Reactivity: Mouse    
Applications: FC    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal Antibody    
Conjugation: PerCP-Cy5.5. Ex:482nm. Em:695nm.
Gene Name: interleukin 7 receptor
Gene Symbol: Il7r
Synonyms: CD127; IL-7Ralpha
Gene ID: 16197
UniProt ID: P16872
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of mouse CD127/IL-7Rα(NP_612564.1).
Dilution: FC,5 μl per 10^6 cells in 100 μl volume
Purification Method: Affinity purification
Concentration: 0.04 mg/ml
Buffer: PBS with 0.09% Sodium azide,0.2% BSA, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-Il7r antibody




Background

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.




Images

Il7r antibodyFlow cytometry: 1×10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Mouse CD127/IL-7Rα mAb (A27439,5 μl/Test,orange line) or PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1×10^6 C57BL/6 mouse splenocytes were surface-stained with PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620,5 μl/Test,blue line) or PerCP/Cyanine5.5 Rabbit anti-Mouse CD127/IL-7Rα mAb (A27439,5 μl/Test,orange line).Non-fluorescently stained cells were used as blank control (red line).



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