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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | HOP homeobox |
| Gene Symbol: | HOPX |
| Synonyms: | HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31 |
| Gene ID: | 84525 |
| UniProt ID: | Q9BPY8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-73 of human HOPX (NP_631957.1). |
| Dilution: | WB 1:10000-1:40000; IHC 1:600-1:6000; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.54mg/ml |
| Buffer: | Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HOPX antibody |
Background
The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene.
Images
 | Western blot analysis of various lysates using HOPX Rabbit PolymAb® (A15537PM) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 90s. |
 | Western blot analysis of various lysates using HOPX Rabbit PolymAb® (A15537PM) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung tissue using HOPX Rabbit PolymAb® (A15537PM) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using HOPX Rabbit PolymAb® (A15537PM) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using HOPX Rabbit PolymAb® (A15537PM) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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