IPO4 Rabbit pAb (20 μl)

IPO4 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15600
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   importin 4
Gene Symbol:   IPO4
Synonyms:   Imp4; IPO4
Gene ID:   79711
UniProt ID:   Q8TEX9
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 728-1081 of human IPO4 (NP_078934.3).
Dilution:   WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   0.94 mg/mL
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-IPO4 antibody




Background

Predicted to enable nuclear import signal receptor activity and nuclear localization sequence binding activity. Involved in DNA replication-dependent chromatin assembly; DNA replication-independent chromatin assembly; and protein import into nucleus. Located in chromatin. Part of protein-containing complex.




Images

IPO4 antibodyWestern blot analysis of various lysates using IPO4 Rabbit pAb (A15600) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of C6 cells using IPO4 Rabbit pAb (A15600) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using IPO4 Rabbit pAb (A15600) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using IPO4 Rabbit pAb (A15600) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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