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| Applications: | IHC, IF/IC , IP, FC, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Clone ID: | 8V8Y10 |
| Immunogen: | None |
| Dilution: | IHC 1:50-1:200; IF/IC 1:50-1:200; FC 1:50-1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells; ChIP 3μg antibody for 10μg-15μg of Chromatin |
| Purification Method: | Protein A/G purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using Rabbit IgG isotype control (AC042) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using Rabbit IgG isotype control (AC042, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
| Confocal imaging of NIH/3T3 cells using Rabbit IgG isotype control (AC042, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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