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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | adenosine deaminase RNA specific |
| Gene Symbol: | ADAR |
| Synonyms: | DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP |
| Gene ID: | 103 |
| UniProt ID: | P55265 |
| Clone ID: | 25GB3090 |
| Immunogen: | A synthesized peptide derived from human ADAR1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#61635). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#61635, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-adenosine deaminase RNA specific antibody (Cat#61635). Adenosine deaminase RNA specific expression in wild-type (WT) and adenosine deaminase RNA specific (ADAR) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-adenosine deaminase RNA specific antibody (Cat#61635, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Adenosine deaminase RNA specific expression in HepG2 cells using anti-Adenosine deaminase RNA specific antibody (Cat#61635, 1:2,000). Green, isotype control; red, Adenosine deaminase RNA specific. |
 | lmmunocytochemical staining of HepG2 cells with Adenosine deaminase RNA specific antibody (Cat#61635, 1:1,000). Nuclei were stained blue with DAPl; Adenosine deaminase RNA specific was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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