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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | CDK5 regulatory subunit associated protein 3 |
| Gene Symbol: | CDK5RAP3 |
| Synonyms: | C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114 |
| Gene ID: | 80279 |
| UniProt ID: | Q96JB5 |
| Clone ID: | 25GB115 |
| Immunogen: | A synthesized peptide derived from human CDK5RAP3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms.
Images
 | Western blotting analysis using anti-CDK5RAP3 antibody (Cat#65198). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDK5RAP3 antibody (Cat#65198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CDK5RAP3 antibody (Cat#65198). CDK5RAP3 expression in wild-type (WT) and CDK5RAP3 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDK5RAP3 antibody (Cat#65198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CDK5RAP3 expression in HepG2 cells using anti-CDK5RAP3 antibody (Cat#65198, 1:2,000). Green, isotype control; red, CDK5RAP3. |
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