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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | centrosomal protein 55 |
| Gene Symbol: | CEP55 |
| Synonyms: | CT111; MARCH; URCC6; C10orf3 |
| Gene ID: | 55165 |
| UniProt ID: | Q53EZ4 |
| Clone ID: | 24GB14080 |
| Immunogen: | Recombinant protein of human CEP55 |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables identical protein binding activity. Involved in cranial skeletal system development; establishment of protein localization; and midbody abscission. Acts upstream of or within mitotic cytokinesis. Located in Flemming body and centrosome. Implicated in multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly.
Images
 | Western blotting analysis using anti-centrosomal protein 55 antibody (Cat#65058). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-centrosomal protein 55 antibody (Cat#65058). Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-centrosomal protein 55 antibody (Cat#65058, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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