Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | charged multivesicular body protein 5 |
| Gene Symbol: | CHMP5 |
| Synonyms: | Spike; Vps60; CGI-34; PNAS-2; C9orf83; HSPC177; SNF7DC2 |
| Gene ID: | 51510 |
| UniProt ID: | Q9NZZ3 |
| Clone ID: | 24GB8030 |
| Immunogen: | Recombinant protein of human CHMP5 |
| Dilution: | WB 1:200-1:1,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008].
Images
 | Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-charged multivesicular body protein 5 antibody (Cat#63819). Charged multivesicular body protein 5 expression in wild-type (WT) and charged multivesicular body protein 5 (CHMP5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Charged multivesicular body protein 5 expression in HepG2 cells using anti-Charged multivesicular body protein 5 antibody (Cat#63819, 1:1,000). Green, isotype control; red, Charged multivesicular body protein 5. |
You may also be interested in:
