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| Reactivity: | Human, Mouse |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cellular retinoic acid binding protein 1 |
| Gene Symbol: | CRABP1 |
| Synonyms: | RBP5; CRABP; CRABPI; CRABP-I |
| Gene ID: | 1381 |
| UniProt ID: | P29762 |
| Clone ID: | 24GB6125 |
| Immunogen: | A synthesized peptide derived from human CRABP1 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CRABP1 encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue.
Images
 | Western blotting analysis using anti-cellular retinoic acid binding protein 1 antibody (Cat#61195). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cellular retinoic acid binding protein 1 antibody (Cat#61195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-cellular retinoic acid binding protein 1 antibody (Cat#61195). Cellular retinoic acid binding protein 1 expression in wild-type (WT) and cellular retinoic acid binding protein 1 (CRABP1) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cellular retinoic acid binding protein 1 antibody (Cat#61195, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of C2C12 cells with anti-Cellular retinoic acid binding protein 1 antibody (Cat#61195, 1:1,000). Nuclei were stained blue with DAPI; Cellular retinoic acid binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm. |
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