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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit |
| Gene Symbol: | DDOST |
| Synonyms: | OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45 |
| Gene ID: | 1650 |
| UniProt ID: | P39656 |
| Clone ID: | 24GB8235 |
| Immunogen: | Recombinant protein of human DDOST |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Images
 | Western blotting analysis using anti-DDOST antibody (Cat#63861). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDOST antibody (Cat#63861, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-DDOST antibody (Cat#63861). DDOST expression in wild-type (WT) and DDOST shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DDOST antibody (Cat#63861, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of DDOST expression in C2C12 cells using anti-DDOST antibody (Cat#63861, 1:1,000). Green, isotype control; red, DDOST. |
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