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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | dipeptidyl peptidase 9 |
| Gene Symbol: | DPP9 |
| Synonyms: | DP9; DPLP9; DPRP2; HATIS; DPP IX; DPRP-2; IMD111 |
| Gene ID: | 91039 |
| UniProt ID: | Q86TI2 |
| Clone ID: | 24GB8170 |
| Immunogen: | Recombinant protein of human DPP9 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized.
Images
 | Western blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#63848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#63848, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#63848). Dipeptidyl peptidase 9 expression in wild type (WT) and dipeptidyl peptidase 9 (DPP9) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#63848, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Dipeptidyl peptidase 9 expression in HepG2 cells using anti-Dipeptidyl peptidase 9 antibody (Cat#63848, 1:1,000). Green, isotype control; red, Dipeptidyl peptidase 9. |
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