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| Reactivity: | Human, Mouse |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | electron transfer flavoprotein subunit alpha |
| Gene Symbol: | ETFA |
| Synonyms: | EMA; GA2; MADD |
| Gene ID: | 2108 |
| UniProt ID: | P13804 |
| Clone ID: | 24GB6605 |
| Immunogen: | Recombinant protein of human ETFA |
| Dilution: | WB 1:2,500-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-electron transfer flavoprotein subunit alpha antibody (Cat#63577). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-electron transfer flavoprotein subunit alpha antibody (Cat#63577, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-electron transfer flavoprotein subunit alpha antibody (Cat#63577). Electron transfer flavoprotein subunit alpha expression in wild type (WT) and electron transfer flavoprotein subunit alpha (ETFA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-electron transfer flavoprotein subunit alpha antibody (Cat#63577, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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