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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | inner membrane mitochondrial protein |
| Gene Symbol: | IMMT |
| Synonyms: | HMP; P87; P89; PIG4; Mic60; PIG52; MINOS2; P87/89; MICOS60 |
| Gene ID: | 10989 |
| UniProt ID: | Q16891 |
| Clone ID: | 24GB14360 |
| Immunogen: | Recombinant protein of human IMMT |
| Dilution: | WB 1:400-1:2,000 FC 1:1,000 IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables RNA binding activity. Involved in cristae formation. Located in mitochondrial inner membrane. Part of MICOS complex.
Images
 | Western blotting analysis using anti-IMMT antibody (Cat#65109). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IMMT antibody (Cat#65109, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-IMMT antibody (Cat#65109). IMMT expression in wild-type (WT) and IMMT shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IMMT antibody (Cat#65109, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of IMMT expression in HepG2 cells using anti-IMMT antibody (Cat#65109, 1:1,000). Green, isotype control; red, IMMT. |
 | Immunocytochemical staining of HepG2 cells with anti-IMMT antibody (Cat#65109, 1:1,000). Nuclei were stained blue with DAPI; IMMT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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