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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | kelch like ECH associated protein 1 |
| Gene Symbol: | KEAP1 |
| Synonyms: | INrf2; KLHL19 |
| Gene ID: | 9817 |
| UniProt ID: | Q14145 |
| Clone ID: | 25GB495 |
| Immunogen: | Recombinant protein of human KEAP1 |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Images
 | Western blotting analysis using anti-KEAP1 antibody (Cat#61257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KEAP1 antibody (Cat#61257, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-KEAP1 antibody (Cat#61257). KEAP1 expression in wild-type (WT) and KEAP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-KEAP1 antibody (Cat#61257, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of KEAP1 expression in HAP-1 cells using anti-KEAP1 antibody (Cat# 61257, 1:2,000). Green, isotype control; red, KEAP1. |
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