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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | leucine rich glioma inactivated 1 |
| Gene Symbol: | LGI1 |
| Synonyms: | EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN |
| Gene ID: | 9211 |
| UniProt ID: | O95970 |
| Clone ID: | 24GB5950 |
| Immunogen: | A synthesized peptide derived from human Lgi1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-LGI1 antibody (Cat#61141). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-LGI1 antibody (Cat#61141). LGI1 expression in wild type (WT) and LGI1 shRNA knockdown (KD) HeLa cells with 20 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LGI1 antibody (Cat#61141, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat# 201, 1:2,0000) respectively. Image was developed using Plus ECL Super Kit (Cat#MR1004). |
 | Flow cytometric analysis of LGI1 expression in HepG2 cells using LGI1 antibody (Cat#61141, 1:2,000). Green, isotype control; red, LGI1. |
 | Immunocytochemical staining of HepG2 cells with anti-LGI1 antibody (Cat#61141, 1:1,000). Nuclei were stained blue with DAPI; LGI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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