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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | proteasome 20S subunit alpha 6 |
| Gene Symbol: | PSMA6 |
| Synonyms: | IOTA; p27K; PROS27 |
| Gene ID: | 5687 |
| UniProt ID: | P60900 |
| Clone ID: | 24GB13270 |
| Immunogen: | Recombinant protein of human PSMA6 |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome.
Images
 | Western blotting analysis using anti-PSMA6 antibody (Cat#64853). Total cell lysates (15 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PSMA6 antibody (Cat#64853). PSMA6 expression in wild-type (WT) and PSMA6 shRNA knockdown (KD) HeLa cells with 15 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PSMA6 antibody (Cat#64853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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