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| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAD17 checkpoint clamp loader component |
| Gene Symbol: | RAD17 |
| Synonyms: | CCYC; R24L; RAD24; HRAD17; RAD17SP |
| Gene ID: | 5884 |
| UniProt ID: | O75943 |
| Clone ID: | 24GB795 |
| Immunogen: | A synthesized peptide derived from human Phospho-Rad17 (S656) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Images
 | Western blotting analysis using anti-Phospho-Rad17 (S656) antibody (Cat#62429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phospho-Rad17 (S656) antibody (Cat#62429). Phospho-Rad17 (S656) expression in wild-type (WT) and RAD17 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-Rad17 (S656) antibody (Cat#62429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Phospho-Rad17 (S656) expression in HAP-1 cells using anti-Phospho-Rad17 (S656) antibody (Cat#62429, 1:2,000). Green, isotype control; red, Phospho-Rad17 (S656). |
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