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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ribophorin I |
| Gene Symbol: | RPN1 |
| Synonyms: | OST1; RBPH1 |
| Gene ID: | 6184 |
| UniProt ID: | P04843 |
| Clone ID: | 24GB1090 |
| Immunogen: | A synthesized peptide derived from human PRibophorin I |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Images
 | Western blotting analysis using anti-Ribophorin I antibody (Cat#62490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ribophorin I antibody (Cat#62490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Ribophorin I antibody (Cat#62490). Ribophorin I expression in wild type (WT) and Ribophorin I shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Ribophorin I antibody (Cat#62490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Ribophorin I expression in HT-1080 cells using anti-Ribophorin I antibody (Cat#62490, 1:2,000). Green, isotype control; red, Ribophorin I. |
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