Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TATA-box binding protein associated factor 15 |
| Gene Symbol: | TAF15 |
| Synonyms: | Npl3; RBP56; TAF2N; TAFII68 |
| Gene ID: | 8148 |
| UniProt ID: | Q92804 |
| Clone ID: | 24GB1830 |
| Immunogen: | A synthesized peptide derived from human TAF15 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Images
 | Western blotting analysis using anti-TAF15 antibody (Cat#62622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TAF15 antibody (Cat#62622). TAF15 expression in wild type (WT) and TAF15 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TAF15 antibody (Cat#62622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TAF15 expression in HepG2 cells using anti-TAF15 antibody (Cat#62622, 1:2,000). Green, isotype control; red, TAF15. |
 | Immunocytochemical staining of HepG2 cells with anti-TAF15 antibody (Cat#62622, 1:1,000). Nuclei were stained blue with DAPI; TAF15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
You may also be interested in:
