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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | tripeptidyl peptidase 2 |
| Gene Symbol: | TPP2 |
| Synonyms: | IMD78; TPP-2; TPPII; TPP-II |
| Gene ID: | 7174 |
| UniProt ID: | P29144 |
| Clone ID: | 24GB2015 |
| Immunogen: | A synthesized peptide derived from human Tripeptidyl peptidase II |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
Images
 | Western blotting analysis using anti-Tripeptidyl peptidase 2 antibody (Cat#62643). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Tripeptidyl peptidase 2 antibody (Cat#62643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Tripeptidyl peptidase 2 antibody (Cat#62643). Tripeptidyl peptidase 2 expression in wild type (WT) and Tripeptidyl peptidase 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Tripeptidyl peptidase 2 antibody (Cat#62643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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