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Recombinant Anti-Ki67 Mouse mAb (100 μl)
| Reactivity: | H & H |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Proliferation marker protein Ki-67 |
Synonyms: | MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen: | Recombinant protein correspondingto Human Ki67 |
Uniprot ID: | P46013 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Product Usage Information
IHC/IF | Human | 1: 500-1: 1000 | ovarian cancer, tonsil |
ICC/IF | Human | 1: 200-1: 800 | HeLa |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Images
| Immunohistochemistry analysis of paraffin embedded human ovarian cancer using ki67 (GB151141) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using ki67 (GB151141) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using ki67 (GB151141) at dilution of 1: 500 |
| ICC analysis of Ki67(GB151141)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |