MAF1 Rabbit pAb (20 μl)

MAF1 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15531
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse    
Applications:   WB, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   MAF1 homolog, negative regulator of RNA polymerase III
Gene Symbol:   MAF1
Synonyms:   MAF1; MAF1 homolog
Gene ID:   84232
UniProt ID:   Q9H063
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MAF1 (NP_115648.2).
Dilution:   WB 1:200-1:2000
Purification Method:   Affinity purification
Concentration:   1.53 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-MAF1 antibody




Background

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.




Images

MAF1 antibodyWestern blot analysis of various lysates using MAF1 Rabbit pAb (A15531) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.



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