PLS1 Rabbit pAb (20 μl)

PLS1 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15303
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IHC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   plastin 1
Gene Symbol:   PLS1
Synonyms:   DFNA76; PLS1
Gene ID:   5357
UniProt ID:   Q14651
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PLS1 (NP_001165783.1).
Dilution:   WB 1:200-1:2000; IHC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   1.61 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-PLS1 antibody




Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.




Images

PLS1 antibodyWestern blot analysis of various lysates using PLS1 Rabbit pAb (A15303) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using PLS1 Rabbit pAb (A15303) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PLS1 Rabbit pAb (A15303) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.



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