Recombinant Anti-160 kDa Neurofilament Medium Mouse mAb (100 μl)

Neurofilament medium polypeptide

Synonyms:

160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium

Immunogen:

Recombinant protein corresponding to Mouse NF-M

Uniprot ID:

P08553

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Cytoplasm, Cytoskeleton, Intermediate filament

Predicted MW. / Observed MW.

96 kDa / 150 kDa

WB

Mouse, Rat

1: 500-1: 2000

brain,spinal cord

IHC/IF

Mouse, Rat

1: 500-1: 1000

brain

WB analysis of 160 kDa Neurofilament Medium(GB15763).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of 160kDa Neurofilament Medium(GB15763).
Sample: Rat Brain 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h.