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| Reactivity: | Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | aggrecan |
| Gene Symbol: | ACAN |
| Synonyms: | AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD; Aggrecan |
| Gene ID: | 176 |
| UniProt ID: | P16112 |
| Clone ID: | 6L4T2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 761-861 of human Aggrecan (P16112). |
| Dilution: | WB 1:1000-1:4000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ACAN antibody |
Background
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
Images
 | Western blot analysis of lysates from Mouse lung, using Aggrecan Rabbit mAb (A11691) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Aggrecan Rabbit mAb (A11691) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse bone marrow using Aggrecan Rabbit mAb (A11691) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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