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| Reactivity: | Human, Mouse |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | adenylate kinase 4 |
| Gene Symbol: | AK4 |
| Synonyms: | AK3; AK 4; AK3L1; AK3L2; AK4 |
| Gene ID: | 205 |
| UniProt ID: | P27144 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.85 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-AK4 antibody |
Background
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.
Images
 | Western blot analysis of various lysates using AK4 Rabbit pAb (A13461) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg AK4 antibody (A13461). Western blot was performed from the immunoprecipitate using AK4 antibody (A13461) at a dilution of 1:1000. |
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