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| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Alpha-methylacyl-CoA racemase |
Synonyms: | 2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, RACE |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human AMACR |
Uniprot ID: | Q9UHK6 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | PC-3, Caco-2 |
IHC/IF | Human | 1: 500-1: 1000 | kidney, liver cancer |
Background
AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).
Images
| Western blot analysis of AMACR (GB121195) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human kidney using AMACR (GB121195) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human liver cancer using AMACR (GB121195) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |