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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ARF GTPase 6 |
| Gene Symbol: | ARF6 |
| Synonyms: | ARF6; ADP-ribosylation factor 6 |
| Gene ID: | 382 |
| UniProt ID: | P62330 |
| Clone ID: | 9E7T9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 76-175 of human ARF6 (P62330). |
| Dilution: | WB 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ARF6 antibody |
Background
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
Images
 | Western blot analysis of various lysates using ARF6 Rabbit mAb (A11485) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Western blot analysis of various lysates using ARF6 Rabbit mAb (A11485) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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