| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | ATPase inhibitor, mitochondrial |
Synonyms: | Atpi, Atpif1 |
Immunogen: | Recombinant protein corresponding to Mouse ATP5IF1 |
Uniprot ID: | O35143 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 12 kDa / 14 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 300-1: 800 | kidney |
Background
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase.Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Images
| WB analysis of ATP5IF1(GB115625). |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |