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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | biogenesis of ribosomes BRX1 |
| Gene Symbol: | BRIX1 |
| Synonyms: | BRIX; BXDC2; BRIX1 |
| Gene ID: | 55299 |
| UniProt ID: | Q8TDN6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human BRIX1 (NP_060791.3). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 2.58 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-BRIX1 antibody |
Background
Enables RNA binding activity. Predicted to be involved in ribosomal large subunit assembly. Located in chromosome and nucleolus.
Images
 | Western blot analysis of various lysates using BRIX1 Rabbit pAb (A14481) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using BRIX1 Rabbit pAb (A14481) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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