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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | CD3 epsilon subunit of T-cell receptor complex |
| Gene Symbol: | CD3E |
| Synonyms: | T3E; TCRE; IMD18; CD3epsilon; CD3E Antigen |
| Gene ID: | 916 |
| UniProt ID: | P07766 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 111-207 of human CD3E (NP_000724.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.43 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-CD3E antibody |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Images
 | Western blot analysis of various lysates using CD3E Antigen Rabbit pAb (A12415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD3E Antigen Rabbit pAb (A12415) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse thymus using CD3E Antigen Rabbit pAb (A12415) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of Jurkat cells using CD3E Antigen Rabbit pAb (A12415) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of RAW264.7 cells using CD3E Antigen Rabbit pAb (A12415) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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