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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | C-X-C motif chemokine receptor 3 |
| Gene Symbol: | CXCR3 |
| Synonyms: | GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R; CXCR3 |
| Gene ID: | 2833 |
| UniProt ID: | P49682 |
| Clone ID: | 3B7Y7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CXCR3 (P49682). |
| Dilution: | WB 1:1000-1:6000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.15 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CXCR3 antibody |
Background
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716).
Images
 | Western blot analysis of lysates from K-562 cells, using CXCR3 Rabbit mAb (A11294) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of paraffin-embedded mouse spleen using CXCR3 Rabbit mAb (A11294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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