DAPK3 Rabbit pAb (20 μl)

DAPK3 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15047
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IHC, IF/IC, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   death associated protein kinase 3
Gene Symbol:   DAPK3
Synonyms:   DLK; ZIP; ZIPK; DAPK3
Gene ID:   1613
UniProt ID:   O43293
Immunogen:   A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAPK3 (NP_001339.1).
Dilution:   WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100
Purification Method:   Affinity purification
Concentration:   0.42 mg/mL
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-DAPK3 antibody




Background

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.




Images

DAPK3 antibodyWestern blot analysis of various lysates using DAPK3 Rabbit pAb (A15047) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using DAPK3 Rabbit pAb (A15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using DAPK3 Rabbit pAb (A15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using DAPK3 Rabbit pAb (A15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DAPK3 Rabbit pAb (A15047) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.



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