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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | flap structure-specific endonuclease 1 |
| Gene Symbol: | FEN1 |
| Synonyms: | MF1; RAD2; FEN-1 |
| Gene ID: | 2237 |
| UniProt ID: | P39748 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:10-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.96 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FEN1 antibody |
Background
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Images
 | Western blot analysis of various lysates using FEN-1 Rabbit pAb (A1175) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using FEN-1 Rabbit pAb (A1175) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse liver using FEN-1 Rabbit pAb (A1175) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of A549 cells using FEN-1 Rabbit pAb (A1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. |
 | Immunofluorescence analysis of HeLa cells using FEN-1 Rabbit pAb (A1175).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. |
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