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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Heat shock protein family A (Hsp70) member 1A |
| Gene Symbol: | HSPA1A |
| Synonyms: | HSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103; HSP70/HSPA1 |
| Gene ID: | 3303 |
| UniProt ID: | P0DMV8 |
| Clone ID: | 1B10F3 |
| Immunogen: | A synthetic peptide of human HSP70/HSPA1 |
| Dilution: | WB 1:2000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.41 mg/ml |
| Buffer: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HSPA1A antibody |
Background
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Images
 | Western blot analysis of various lysates using HSP70/HSPA1 Mouse mAb (A1507) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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