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Recombinant Anti-His Tag Rabbit mAb (100 μl)
| Reactivity: | |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | His Tag |
Synonyms: | 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED |
Immunogen: | KLH conjugated Synthetic peptide corresponding to HHHHHH (6* His) |
Isotype: | IgG |
Purity: | Affinity purification |
Product Usage Information
WB | Species independent | 1: 2000-1: 5000 | Transfected HEK-293F cells |
ICC/IF | Species independent | 1: 500-1: 1000 | Transfected HEK-293F cells |
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper.
Images
| WB analysis of His Tag (GB151255). |
| ICC analysis of His Tag (GB151255) red. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |