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| Reactivity: | Human, Mouse |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | integrator complex subunit 5 |
| Gene Symbol: | INTS5 |
| Synonyms: | INT5; KIAA1698; INTS5 |
| Gene ID: | 80789 |
| UniProt ID: | Q6P9B9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human INTS5 (NP_085131.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.97 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-INTS5 antibody |
Background
The Integrator complex is a complex that associates with the C-terminal domain of RNA polymerase II large subunit. This complex is brought to U1 and U2 small nuclear RNA genes, where it is involved in the transcription and processing of their transcripts. The protein encoded by this gene represents a subunit of the Integrator complex.
Images
 | Western blot analysis of various lysates using INTS5 Rabbit pAb (A13646) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus using INTS5 Rabbit pAb (A13646) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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