Reactivity: | M,R & H,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | MT-ND5 rabbit polyclonal antibody |
Gene Name: | NADH-ubiquinone oxidoreductase chain 5 |
Synonyms: | NADH dehydrogenase subunit 5, Mtnd5, mt-Nd5, Nd5 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MT-ND5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 68 kDa |
Observed MW. | 68 kDa |
Uniprot ID: | P03921, P11661 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1:1000-1:2000 | liver, brain |
IHC/IF | Human, Rat | 1:400-1:800 | heart, kidney, lung, lung cancer |
Background
ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Images
![]() | Western blot analysis of MTND5 (GB112639) at dilution of 1: 1000 |
Immunofluorescence of paraffin embedded rat lung using MTND5 (GB112639) at dilution of 1: 400 (400x lens) | |
Immunofluorescence of paraffin embedded human lung cancer using MTND5 (GB112639) at dilution of 1: 400 (450x lens) | |
![]() | Immunofluorescence of paraffin embedded rat heart using MTND5 (GB112639) at dilution of 1: 400 (400x lens) |
Immunofluorescence of paraffin embedded rat kidney using MTND5 (GB112639) at dilution of 1: 400 (450x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |