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| Reactivity: | Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | myosin heavy chain 13 |
| Gene Symbol: | MYH13 |
| Synonyms: | MyHC-eo; MyHC-IIL; MYH13 |
| Gene ID: | 8735 |
| UniProt ID: | Q9UKX3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1240-1320 of human MYH13 (NP_003793.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.81 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-MYH13 antibody |
Background
Predicted to enable microfilament motor activity. Predicted to be involved in muscle contraction. Predicted to act upstream of or within cellular response to starvation. Located in extracellular exosome.
Images
 | Western blot analysis of various lysates using MYH13 Rabbit pAb (A14620) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of paraffin-embedded rat eye using MYH13 Rabbit pAb (A14620) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of paraffin-embedded mouse eye using MYH13 Rabbit pAb (A14620) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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