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Anti-N Cadherin Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Cadherin-2 |
Synonyms: | Neural cadherin, N-cadherin, CD325, CDw325,Cadherin 2 N cadherin neuronal, CDHN, Cdh2, Cadherin 2 type 1, NCAD, N cadherin 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse N-cadherin |
Uniprot ID: | P15116 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cell membrane,Cell junction,Cell surface |
Predicted MW. / Observed MW. | 100 kDa / 140 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1:1000-1:2000 | colon, brain, cerebral cortex |
IHC/IF | Human, Mouse, Rat | 1:500-1:2000 | colon |
Background
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Images
| Western blot analysis of N-Cadherin (GB12135) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human colon using N Cadherin (GB12135) at dilution of 1: 1500 |
| Immunohistochemistry analysis of paraffin-embedded mouse colon using N Cadherin (GB12135) at dilution of 1: 1500 |
| Immunohistochemistry analysis of paraffin-embedded rat colon using N Cadherin (GB12135) at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |