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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | neutrophil cytosolic factor 1 |
| Gene Symbol: | NCF1 |
| Synonyms: | CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A; p47phox/NCF1 |
| Gene ID: | 653361 |
| UniProt ID: | P14598 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human p47phox/NCF1 (NP_000256.4). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.16 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-NCF1 antibody |
Background
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Images
 | Western blot analysis of various lysates, using p47phox/NCF1 Rabbit pAb (A1148) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from Raji cells using p47phox/NCF1 Rabbit pAb (A1148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:10s. |
 | Immunoprecipitation analysis of 200 μg extracts of Raji cells, using 3 μg p47phox/NCF1 antibody (A1148). Western blot was performed from the immunoprecipitate using p47phox/NCF1 antibody (A1148) at a dilution of 1:1000. |
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